BACKGROUND: High fat diets are known to cause a positive fat balance and
consequently to the accumulation of adipose mass, this diet does not seem to
stimulate fat oxidation in the same way in obese and lean subjects. HFD was an
inducing factor for ICAM-1 expression in the aorta of Wistar rats. HFD effect on
ICAM-1 seems to be time dependent. ICAM-1 is one of the first events in the
formation of atherosclerotic lesions. HFD up-regulated Cav-1, regulated
expression other biomarker in HFD is eNOS.
METHOD: Study design was experimental study, by used Randomized Post Test
only Control Group Design with Kruskal-Wallis test was used to analyze the
differences among groups and followed by a Mann Whitney test. Treatment is
ethanolic of Eurycoma longifolia Jack, and out come are sICAM-1 and eNOS
levels. Thirty Sprague Dawley (SD) Rat, were divided into 6 groups. C(-) was SD
group, C(+) was group with HFD, X1 (SD treated with EL dosage 10 mg/kg), X2
(SD treated with EL dosage 15 mg/kg), X3 (HFD treated with EL dosage 10
mg/kg), X4 (HFD treated with EL dosage 15 mg/kg).
RESULT: No significant difference in ICAMs that was found among the studied
groups (p = 0.087). Significant difference in eNOS among six group studied (p =
0.033). The control negative group was not significance different than control
positive, X2, X3, and X4 (p = 0.841; p = 0.310; p = 0.056; p = 0.095),
respectively.
CONCLUSION: Non significant difference on level of ICAM-1 was found
among groups with or without HFD treatment. non Significant difference on the
level of eNOS was found among groups with or without HFD treatment.
Keywords: Eurycoma longifolia Jack, sICAM, eNOS, HFD.